Modification of the silver staining technique to detect lipopolysaccharide in polyacrylamide gels
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چکیده
منابع مشابه
Modification of the silver staining technique to detect lipopolysaccharide in polyacrylamide gels.
A silver staining method used routinely for detecting bacterial lipopolysaccharide (LPS) in sodium dodecyl sulfate-polyacrylamide gels (C. Tsai and E. Frasch, Anal. Biochem. 119:115-119, 1982) appeared to be inappropriate for visualizing certain LPS preparations. It did not stain S-form fractions of polyagglutinable Pseudomonas aeruginosa LPS or several partly deacylated (alkali-treated) S-form...
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On the basis of the physico-chemical principles underlying silver-staining of proteins, which are recalled in this paper, several methods of silver-staining of proteins after SDS electrophoresis in polyacrylamide gels or isoelectric focusing were tested. The most valuable protocols are presented in this report, including standard methods for unsupported gels and new methods devised for thin (0....
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Silver-staining of nucleic acid has been used for various biological analyses, including polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis. A variety of methods have been described, but these methods are not that effective for staining more than a few PCR-SSCP gels, especially rapidly and with high sensitivity, because they include a number of time-consuming ...
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The classic silver staining method for detecting bacterial lipopolysaccharides (LPSs) in polyacrylamide gels (C. Tsai and C. E. Frasch, Anal. Biochem. 119:115-119, 1982) was at least 20 times more sensitive than the modified silver staining method (A. Fomsgaard, M. A. Freudenberg, and C. Galanos, J. Clin. Microbiol. 28:2627-2631, 1990) for detecting LPS from the bacterium Moraxella osloensis. H...
متن کامل1 Silver staining of proteins in polyacrylamide gels : a general overview
(Running title): overview of silver staining methods for proteins ABSTRACT On the basis of the physico-chemical principles underlying silver staining of proteins, which are recalled in this paper, several methods of silver staining of proteins after SDS electrophoresis in polyacrylamide gels or isoelectric focusing were tested. The most valuable protocols are presented in this report, including...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1990
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.28.12.2627-2631.1990